Studies on Transfer Ribonucleic Acids and Related Compounds. XXXI. Synthesis of the 3', 5'-Bisphosphorylated Hexanucleotide corresponding to Bases (72-77) of tRNA^fMet from E. coli and Its Base Transition Analog

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A hexanucleotide pC-A-A-C-C-Ap, which corresponds to the 3'-end of tRNA^fMet, was synthesized as a bisphosphorylated form, which should be a suitable donor substrate for joining with RNA ligase. 3'-Phosphorylated oligonucleotides were condensed by a triester method and the 5'-terminus of the protected hexanucleotide was phosphorylated with dianilidophosphorochloridate after demonomethoxytritylation. A site-directed base transition mutant sequence C-G-A-C-C-Ap was also synthesized by the triester method using suitably protected 3'-phosphorylated oligonucleotides.