Quantitative Analysis of the Isomers of Hydroxyphenylalanine by High-Performance Liquid Chromatography using a Fluorimetric Detector

Abstract

A sensitive high-performance liquid chromatographic technique for the quantitative analysis of the isomers of hydroxyphenylalanine has been developed. The chromatographic conditions were as follows : stainless steel column (2.1×500 mm) packed with Hitachi #3011-C resin ; mobile phase, a mixture of equal volumes of 0.025 M sodium acetate and 0.05 M acetic acid ; flow rate, 0.8 ml/min ; temperature, 45° ; detection, fluorimetric detector. This procedure was found to be suitable for the analysis of 3, 4-dihydroxyphenylalanine, p-tyrosine, m-tyrosine, o-tyrosine, and phenylalanine.