Abstract
An active principle having gastric juice secretion-inhibiting activity in pylorus-ligated rats was purified from the ethanol extract of dried brewer's yeast by column chromatography, preparative thin-layer chromatography on silica gel, and gel filtration on a Sephadex LH-20. The active principle was a fatty acid mixture, consisting of C10 : 0, C12 : 0, C14 : 0, C15 : 0, C16 : 0, C16 : 1 (palmitoleic acid), C18 : 0, C18 : 1 (oleic acid), and C18 : 2 (linoleic acid) as determined by gas chromatography-mass spectrometry of the methyl esters. The biological activity of each identified fatty acid on gastric juice secretion was assayed in pylorus-ligated rats at a dose of 100 mg/kg (i. p.); the C10 : 0, C12 : 0, C14 : 0, C15 : 0, and C16 : 0 acids markedly decreased gastric juice volume, total acid output, and total peptic activity. Gastric volume and total acid output were significantly inhibited by C16 : 1 acid, and the gastric volume was inhibited by C18 : 2 acid. Among the C10 : 0, C12 : 0, C14 : 0, C15 : 0, and C16 : 0 acids, the C12 : 0 and C14 : 0 acids significantly prevented ulcer formation in pylorus-ligated rats.
