A Sensitive Gas Chromatographic Assay for Cystathionase and Cystathionine : Application of This Assay to Biological Samples and Kinetic Studies. Gas Chromatographic Assay for Cystathionase and Cystathionine

Abstract

α-Ketobutyric acid is one of the reaction products formed from cystathionine or homoserine by cystathionase. α-Ketobutyric acid was reacted with pentafluorophenylhydrazine, and the hydrazone was extracted with ethyl ether, reacted with diazomethane, and subjected to gas-liquid chromatography with an electron capture detector. This method provides a specific and sensitive assay for cystathionase and cystathionine in biological samples.