1982 Volume 30 Issue 6 Pages 2105-2109
A procedure for the determination of the heat-stable alkaline phosphatase activity in serum was developed by using antibody-conjugated paper disks and inhibition by sodium thiocyanate. The placental alkaline phosphatase adsorbed on a paper disk conjugated with antibody was completely inhibited by 3M sodium thiocyanate, but the activity of intestinal alkaline phosphatase adsorbed on the paper disk was unaffected by 3M sodium thiocyanate. The results obtained by the proposed method showed a good correlation with those obtained by the heat-inactivation method (γ=0.991). These results strongly suggest that the proposed method can be utilized as a routine clinical test for the determination of serum placental-type alkaline phosphatase, i. e., placental, Regan, Nagao and Kasahara isoenzymes, from patients with cancer.
