Preventive Effect of 2-Halogen-Substituted Derivatives of Cyclic Adenosine 3', 5'-Monophosphate on Experimental Disseminated Intravascular Coagulation

Abstract

Cyclic adensine 3', 5'-monophosphate (cAMP) derivatives were tested for inhibitory effect on the development of experimental disseminated intravascular coagulation (DIC). Two compounds, 2-Cl-cAMP and 2-Br-cAMP, which inhibited the platelet aggregation induced by collagen in vitro, had a strong inhibitory effect on the development of experimental DIC in rats in vivo as judged by five diagnostic laboratory tests, prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrin and fibrinogen degradation products (FDP), fibrinogen and platelet count. The effect of these derivatives was preventive rather than therapeutic, and the inhibition of rat platelet aggregation by these substances was marked when the stimulator was collagen or thrombin but less strong when it was calcium ionophore A23187 or adenosine 5'-diphosphate (ADP).