IN SITU DETECTION OF HISTAMINE RELEASED FROM MAST CELLS BY USING A HISTAMINE-SENSITIVE MEMBRANE ELECTRODE

Abstract

We constructed a histamine-sensitive membrane electrode by using sodium tetrakis(p-fluorophenyl)borate as an ion-exchanger and o-nitrophenylphenylether as a membrane solvent and applied it in the detection of histamine released from mast cells. This is the first attempt to measure the histamine secretion process in situ without any separation of histamine from the assay medium.