1987 Volume 35 Issue 1 Pages 416-420
The effect of lipopolysaccharide (LPS) on the urinary excretion of salicylamide (SAM) (10 mg/head) given orally to rats was examined 24 h after intraperitoneal administration of LPS (0.5 mg/kg) by the direct analysis of urine samples by high-performance liquid chromatography. The cumulative amount of SAM glucuronide excreted in urine in 4 h was decreased by 40-70% in the LPS-administered rats, while that of SAM sulfate was increased by 7-15% compared with the control in the same rats. The amounts of the conjugates of gentisamide (the hydroxylated metabolite of SAM) excreted in urine were decreased by approx. 70% compared with the control. LPS administration provoked a decrease in the activities of aniline hydroxylase of liver and uridine diphosphate (UDP) -glucuronyltransferase of liver, lung and kidney, but did not change the activities of sulfotransferase of liver or UDP-glucuronyltransferase obtained from the small intestine. The mechanism of the decreased urinary excretion of SAM glucuronide in LPS-administered rats is discussed.
