Subculture of bovine endothelial cells with 33 μm vitamin D3 or 25-(OH)-D3 for 48 h caused marked morphological change. Angiotensin converting enzyme (ACE) activity of the morphologically changed cells was decreased to approximately one-third and plasminogen activator (PA) activity released from them increased approximately 12-fold as compared to the control cells. Fibrin autography revealed both urokinase-type (53kDa) and tissue-type (80kDa) PAs in the culture medium of the cells treated with vitamin D3 Neither 1, 25-(OH) 2-D3 nor 24, 25-(OH) 2-D3 had any effect on PA activity released from the cells, while ACE activity of the cells was decreased by the treatment with these dihydroxylated vitamins. Provitamin D3 or cholesterol had no effect on PA or ACE activities of endothelial cells.
The Pharmaceutical Society of Japan