Abstract
Eight dodecauncleotides, each containing deoxynosine or deoxyguanosine at a pairing position to one of the four major deoxynuclesides, d(CGCIAATTNGCG)or d(CGCGAATTNGCG)(N=dA, dC, dG and dT), were synthesized by the phosphotriester method in solution. Purification methods which were suitable for milligram quantities of oligonucleotides were established.The thermal stabilities of these duplexes and the circular dichroism spectra suggested that the dodecamers formed duplexes similar to B-deoxyribonucleic acid.
