Synthesis of Wobble Pairing Oligoribonucleotides

Abstract

Hexaribonucleotides containing inosine at a pairing position adjacent to the other four major nucleosides (GGINCC, N=A, C, G, U) were synthesized in solution by the phosphotriester method using 2'-O-tetrahydrofuranyl protecting groups. 5'-O-Dimethoxytrityl-2'-O-tetrahydropyranylinosine 3'-O-(metyl-N, N-diisopropyl)phosphor-amidite was used for solid-phase syntheses of decaribonucleotides (CGNGAUCICG, N=A, C, G, U). Thermal stabilities and circular dichroism spectra for these self-complementary duplexes were measured. The stabilizing effects of wobble base pairs in these duplexes were estimated as IC>IA>IU>IG.