1990 Volume 38 Issue 3 Pages 724-727
Methods employing colorimetry and high-performance liquid chromatography (HPLC) were developed for determination of D-glucosone. Colorimetry with phenazine methosulfate and nitro blue tetrazolium as electron acceptors made it possible to determine D-glucosone (sensitivity : 50 nmol) in the presence of excess amounts of D-glucose and D-fructose. Further, by using a high-performance liquid chromatograph equipped with a ligand-exchange mode column, we could separate D-glucosone in human and rat sera from D-glucose. The blood level of D-glucosone 5 min after intraperitoneal administration (1.68 mmol/kg) of the sugar to rats was determined by the HPLC method to be 1.3 mM in portal blood and 0.5 mM in postcaval blood. The maximum increase in the blood glucose level was observed 2 h after administration of the D-glucosone. A similar increase of blood glucose was observed after the administration of 2-deoxy-D-glucose, and seemed to continue until 5 h. The assay methods described are useful for biochemical studies on D-glucosone as an analogue of D-glucose.
