Enzyme Immunoassay for the Drug of Anti-ulcer Using Avidin-Biotin System

Abstract

We have developed a competitive enzyme immunoassay for a drug, which was a newly synthesized anti-ulcer agent, using an enzyme immunoassay. The polyclonal anti-drug antibody coupled to biotin, peroxidase labeled drug derivatives as a tracer, and a small column of Sepharose 4B covalently bound to avidin were used in the assay. This assay is simple and rapid, and the sensitivity and the measuring range can be controlled by the flow rate of the substrate solution. The correlation between serum drug concentrations (0.1-10μg/ml) measured by gas chromatography and this assay was good (r=0.991). This principle for the assay is very practical and applicable to the enzyme immunoassay for small and large molecules.