1994 Volume 42 Issue 2 Pages 395-397
An assay method for the determination of curculigoside in Curculiginis Rhizoma by high performacne liquid chromatography (HPLC) was set up. After extraction with ethyl acetate, curculigoside was hydrolyzed in 1 N NaOH, and quantitatively converted to 2, 6-dimethoxybenzoic acid (2, 6-DA). The determination of curculigoside in Curculiginis Rhizoma was performed indirectly by measuring the content of 2, 6-DA by HPLC. The calibration curve for this method exhibited a good linearity with a correlation coefficient of 1.0000 over the concentration range 1.0 to 81.0 μg 2, 6-DA/ml (from 3 to 207 μg/ml as curculigoside). Using this method, 7 lots of Curculiginis Rhizoma produced in China contained about 0.2% curculigoside on average. To estimate the reliability of this method, the curculigoside content of Curculiginis Rhizoma was determined by direct assay applying the HPLC method and using curculigoside as the standard. Both methods agreed very well and this method was found to be satisfactory for estimating the contents of curculigoside in Curculiginis Rhizoma.
