Abstract
Suspended 3T3 cells are highly resistant to immune cytolysis with antibodies and complement. This lytic resistance was not ascribed to the loss of surface antigens in the suspended 3T3 cells as demonstrated in a previous report (6). When treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), however, 3T3 cells in suspension became highly sensitive to immune cytolysis and decreased in cell-aggregability. A concomitant observation was that most MNNG-treated cells had morphologically altered microvilli. This indicates that the occurrence of microvilli is related to the resistance to immune cytolysis of the suspended 3T3 cells.
