Flow Cytometry for Rapid Detection of Ampicillin Activity Using Bacterial Susceptibility and its Application to Aquaculture

抄録

Rapid detection of ampicillin activity by flow cytometry (FCM) technique was described. The FCM scattergram for bacterial cells exposed to ampicillin was changed with incubation time. The phenomenon might be reflected by changes in bacterial cell size and the structure of envelope induced by ampicillin activity. The technique of FCM was applied for the detection of ampicillin activity in samples for aquaculture. A good correlation was observed between the values measured by FCM and conventional method. One FCM assay could be completed within 60 s and the total assay time including the preparation of samples was within 2.5 h.