Endocrine Journal
Online ISSN : 1348-4540
Print ISSN : 0918-8959
ISSN-L : 0918-8959

この記事には本公開記事があります。本公開記事を参照してください。
引用する場合も本公開記事を引用してください。

Expression and mechanism of microRNA 195 in diabetic retinopathy
Li ShanHaiyong ZhangYing HanRuijuan Kuang
著者情報
ジャーナル フリー 早期公開

論文ID: EJ21-0231

この記事には本公開記事があります。
詳細
抄録

We aimed to explore the role of microRNA 195 (miR-195) in diabetic retinopathy (DR). From January 2019 to July 2020, 50 patients with DR undergoing vitrectomy and 40 patients with idiopathic macular holes undergoing vitrectomy were selected as the observation group (OG) and control group (CG), respectively. The mRNA and protein expression levels of miR-195, SIRT1, BAX, and BCL-2 were detected in the retinal tissues obtained from the two groups during surgery. In addition, human retinal endothelial cells and human dermal microvascular endothelial cells were cultured in a high-glucose environment to detect the targeted relationship between miR-195 and SIRT1; determine the mRNA and protein expression levels of SIRT1, BAX, and BCL-2 after miR-195 knockdown; and assess the levels of cell proliferation and apoptosis. In OG, the mRNA and protein expression levels of miR-195 and BAX were high, whereas those of BCL-2 and SIRT1 were low. Moreover, we detected a targeted relationship between miR-195 and SIRT1. Conversely, miR-195 knockdown led to the downregulation of the mRNA and protein expression levels of BAX and the upregulation of the mRNA and protein expression levels of SIRT1 and BCL-2 as well as improvement in cell growth and a decrease in the apoptosis rate. miR-195 is ‍overexpressed in DR, and its targeted relationship with SIRT1 inhibits the growth of cells in the retina and accelerates ‍apoptosis.

著者関連情報
© The Japan Endocrine Society
feedback
Top