抄録
1. The effects of Parotin on the development of 7-and 9-day chick embryo femora were studied in tissue culture.
2. Pair-mate explants were cultivated by roller-tube methods with use of slightly modified “dynamic medium” and demonstrated a histological and anatomical development. One of each pair was cultured in the plus-Parotin medium with various concentrations.
3. These experiments resulted in the following;(a) in the concentration of 15mg per cent region in the medium, it was found Parotin to have stimulative action on the longitudinal growth of the explants, (b) in the same concentration promotive effect on the deposition of calcium was observed when 9-day chick embryo femora was employed, (c) in a higher concentration, inhibitory effect was found remarkably in both processes of ossification.
4. Accordingly it was expected that Parotin might have direct stimulative effects on some sites of development of hard tissues in vivo.
