The author has observed the response of isolated thoracic ducts of cows against various chemicals by Magnus's method. for the purpose of determining the structu ral function of the wall of thoracic duct and arrived at the following results : 1) The thoracic duct is capable of spontaneous movement, but moves in such a manner only on rare occasions. The spontaneous movement then is in the form of a periodical pendulum movement. 2) The tension of isolated thoracic duct is accentuated by sympathetic as well as parasympathetic nerve stimulants showing no antagonism between these two sorts of stimulants. This fact may be easily interpreted upon considering the histological structure of thoracic duct. Be the two kinds each of the adventitial and the medial muscle bundles under the control of either the sypathetic or the parasympathetic nerves, it is quite understandable that the tension rises with use of stimulants and falls with the use of either sympathetic or parasympathetic depressants as shown in the author's experiments.