Heat Resistance of Acanthamoeba sp. Cysts in Green Mussel Broth and Phosphate-Buffered Saline

Abstract

Cysts propagated by the agar block method with heat-killed Escherichia coli as nutrient overlay were inoculated into Perna viridis broth (PVB) and phosphate-buffered saline (PBS) prior to exposure to 60, 75 and 100°C for 0, 3, 5, and 10 min. The heat resistance of Acanthamoeba sp. cysts expressed in of D- and Z- values were found to be greater in the complex organic PVB than in the aqueous PBS. The established D- values in the PVB were 81.20, 44.59, and 8.83 min at 60, 75, and 100°C, respectively. The calculated Z-value of Acanthamoeba sp. cysts in PVB was 40.28°C.