Abstract
Debaryomyces hansenii NBRC 0855 accumulated high amounts of glucosylceramide relative to 25 strains of salt-tolerant yeasts in the genera Candida, Debaryomyces, Kodamaea, and Pichia. The constituents of glucosylceramide were principally 2-hydroxystearic acid as a fatty acid, 9-methyl-trans-4, trans-8-sphingadienine as a sphingoid base and glucose as a hexose. Strain NBRC 0855 was able to produce glucosylceramide when cultured on actual effluent from a pickle manufacturer.