Food Science and Technology Research
Online ISSN : 1881-3984
Print ISSN : 1344-6606
ISSN-L : 1344-6606
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Loss of poly-γ-glutamic Acid Synthesis of Bacillus subtilis (natto) Due to IS4Bsu1 Translocation to swrA Gene
Keitarou KIMURALam-Son Phan TRANKazumi FUNANE
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2011 Volume 17 Issue 5 Pages 447-451

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Abstract

The mobile genetic element IS4Bsu1 of Bacillus subtilis (natto), the commercial strain used for natto fermentation, was found to spontaneously translocate to the swrA gene, which led to a defect in poly-γ-glutamic acid (γPGA) synthesis. The γPGA defect of NAFIS1 (swrA::IS4Bsu1) was restored by a plasmid harboring the swrA gene. The insertion of IS4Bsu1 to the swrA gene appeared not to interfere with minJ gene expression, located downstream of the swrA gene, because a defect in cell motility exhibited by NAF16 (minJ::Spcr) was not observed in NAFIS1 (swrA::IS4Bsu1). We found that the minJ gene is essential for γPGA synthesis.

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© 2011 by Japanese Society for Food Science and Technology
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