Isolation and Characterization of Pepsin-Soluble Collagen from Abalone (Haliotis discus hannai) Gastropod Muscle Part II

Xiuping DONG; Qixin YUAN; Hang QI; Jingfeng YANG; Beiwei ZHU; Dayong ZHOU; Yoshiyuki MURATA; Wenxiu YE

doi:10.3136/fstr.18.271

Original papers

Isolation and Characterization of Pepsin-Soluble Collagen from Abalone (Haliotis discus hannai) Gastropod Muscle Part II

Xiuping DONG, Qixin YUAN, Hang QI, Jingfeng YANG, Beiwei ZHU, Dayong ZHOU, Yoshiyuki MURATA, Wenxiu YE

Author information

Xiuping DONG
School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, Special Sub-center of Shellfish of National Research and Development Center of Agriculture Product Processing Technology of Ministry of Agriculture
Qixin YUAN
School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, Special Sub-center of Shellfish of National Research and Development Center of Agriculture Product Processing Technology of Ministry of Agriculture
Hang QI
School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, Special Sub-center of Shellfish of National Research and Development Center of Agriculture Product Processing Technology of Ministry of Agriculture
Jingfeng YANG
School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, Special Sub-center of Shellfish of National Research and Development Center of Agriculture Product Processing Technology of Ministry of Agriculture
Beiwei ZHU
School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, Special Sub-center of Shellfish of National Research and Development Center of Agriculture Product Processing Technology of Ministry of Agriculture
Dayong ZHOU
School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, Special Sub-center of Shellfish of National Research and Development Center of Agriculture Product Processing Technology of Ministry of Agriculture
Yoshiyuki MURATA
Department of biological resources chemistry, faculty of agriculture, Okayama University
Wenxiu YE
School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, Special Sub-center of Shellfish of National Research and Development Center of Agriculture Product Processing Technology of Ministry of Agriculture

Keywords: abalone (Haliotis discus hannai), gastropod muscle, pepsin-soluble collagen, characterization

JOURNAL FREE ACCESS

2012 Volume 18 Issue 2 Pages 271-278

DOI https://doi.org/10.3136/fstr.18.271

Details

Abstract

Abalone (Haliotis discus hannai) gastropod muscle was divided into Part I (the center part of gastropod muscle) and Part II (the transforming and virginal part of gastropod muscle). Pepsin-soluble collagen was extracted from abalone gastropod muscle part II (PSCAGM-II) with yield of 8.7%. SDS-PAGE and amino acid composition indicated that the PSCAGM-II contained α₁ and α₂ chains and might be classified as type I collagen. PSCAGM-II exhibited a maximum absorbance at 232 nm, but little absorbance near to 280 nm. FTIR investigations showed the existence of helical arrangements of collagen. PSCAGM-II triple helical stereochemical structure was destroyed when the treated temperature up to 35°C for 10 min. Denatured temperature detected by the viscometer was 22.9°C when incubated for 20 min. Study on the properties and thermal stability of PSC from abalone gastropod muscle has certain directive significance for the abalone processing industry.

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