2017 年 23 巻 6 号 p. 863-870
The aim of this study was to investigate the effects of organic acids, sugars, and oils used as food additives on histamine production by a halotolerant histamine-producing bacterium, Staphylococcus epidermidis TYH1, isolated from fermented fish paste. The test strain was incubated in LB medium (pH 5.0) containing 0.5% histidine and various concentrations of organic acids, sugars, or oils. TYH1 proliferated and produced significant amounts of histamine in the medium containing 1–10% (w/v) glucose or soybean oil. Histamine production was markedly accelerated in the medium with 30 mM acetic acid, 30 mM malic acid, and 10 mM citric acid. However, histamine accumulation was suppressed by the addition of higher concentrations of organic acids to the medium. The minimum inhibitory concentrations (MICs) of acetic, malic, citric and lactic acids for both histamine accumulation in the medium and proliferation of TYH1 were 80, >100, 30, and >100 mM, respectively. These findings may contribute to the development of techniques to prevent histamine food poisoning.
