Optimization of a Molasses Based Fermentation Medium for Lipases from Burkholderia sp. Bps1 Based on Response Surface Methodology

Abstract

This study aimed to acquire a low-cost medium to produce high-yields of lipases from Burkholderia sp. Bps1 by using a statistic method so as to optimize the compositions of medium in the following two steps: 1) a single factor experiment (SFE) was conducted to screen the most significant factors affecting lipase activity to determine the optimal carbon source (molasses), nitrogen source (peptone) and inducer (palm oil); 2) a central composite design (CCD) and response surface methodology (RSM) were used to further optimize the previously mentioned factors to acquire an optimal combination consisting of 9.7 g/L molasses, 13.7 g/L peptone and 0.60% (v/v) palm oil. The optimized lipases activity reached 153.537 U/mL which was 30 times greater than the activity of the primary enzyme. This research used a statistical method to improve the production of lipases from submerged fermentation and formed a theoretical foundation for the industrial production of lipases using low cost raw materials.