2020 Volume 26 Issue 5 Pages 611-621
Two new types of hydro soluble polysaccharides, denoted AMP-1 and AMP-2 were first extracted from the stalk of Abelmoschus manihot (L.) Medic by DEAE Sephadex A-52 chromatography and Sephadex G-100 chromatography in sequence. The structure characterization and monosaccharide composition were analyzed by SEM, DSC, FT-IR and GC. The polysaccharide fractions had good thermal stability, while possessing significant variance in surface features. Meanwhile, it was found that the two polysaccharides contained different monosaccharide compositions with diverse molar ratios. The antioxidant assays manifested that AMP-1 and AMP-2 showed 1, 1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radicals and superoxide anion radicals scavenging activities. In addition, the polysaccharides possessed the ability to bind to sodium cholate, sodium deoxycholate and sodium taurocholate. These results suggested that AMP-1 and AMP-2 have broad prospects in the development of value enhancement products with hypolipidemic and free radical inhibitor activity.
