Preparation of a Bispecific Antibody to Ovomucoid and Horseradish Peroxidase by Chemical Recombination Method

Abstract

Antibodies capable of binding two different antigens are termed bispecific antibodies. In this study, we prepared a bispecific antibody reactive to ovomucoid and horseradish peroxidase by a chemical recombination method. Using the resulting F(ab')₂ type bispecific antibody, we obtained a good standard curve of ovomucoid by a competitive ELISA.