Food Science and Technology Research
Online ISSN : 1881-3984
Print ISSN : 1344-6606
ISSN-L : 1344-6606
Notes
Apoptosis-Inducing Effect of Fucoxanthin on Human Leukemia Cell Line HL-60
Masashi HOSOKAWASatoshi WANEZAKIKozue MIYAUCHIHideyuki KURIHARAHiroyuki KOHNOJun KAWABATAShizuo ODASHIMAKoretaro TAKAHASHI
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1999 Volume 5 Issue 3 Pages 243-246

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Abstract

The apoptosis-inducing effect of fucoxanthin on human leukemic HL-60 cells was investigated. Fucoxanthin, which was obtained from the brown alga Undaria pinnatifida, inhibited the proliferation of HL-60 cells and induced DNA fragmentation on agarose gel electrophoresis, a typical characteristic feature of apoptotic cells. The results of sandwich ELISA using anti-biotin-antibody and anti-DNA-antibody also demonstrated DNA fragmentation in accordance with the increase in fucoxanthin concentration and incubation time. Since the level of DNA fragmentation did not increase after 24 h incubation, fucoxanthin appeared to have some effect on cell cycle. In contrast, β-carotene did not show an apoptosis-inducing effect on HL-60 cells. These results suggested that a carotenoid structure might be crucial for inducing apoptosis.

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© 1999 by Japanese Society for Food Science and Technology
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