Apparent Distribution Coefficients of Glucose and Fructose onto Cation-Exchange Resins in Calcium-Ion Form with Different Divinylbenzene Contents

Abstract

The apparent distribution coefficients, K_app, of glucose and fructose onto cation-exchange resins in calcium-ion form with different divinylbenzene (DVB) contents were observed over a wide concentration ranges. Irrespective of the DVB content of the resin, the K_app value of glucose was larger with an increase in the glucose concentration, while the K_app value of fructose was smaller at higher fructose concentrations. A method for estimating the binding constant of fructose to the calcium-ion, B_Fru, was proposed under the assumption that the constant of glucose could be approximated as zero, and the B_Fru value was evaluated as 0.66 l/mol. The K_app values of mannose onto a resin with a DVB content of 4% were also measured at various solute concentrations, and the B_Man value was estimated to be 0.31 l/mol.