Abstract
The objective of this study was to evaluate the effects of high-molecular-weight glutenin subunits with different protein contents on the quality of flour. The flour properties of near isogenic lines (NILs), which were substituted with HMWG subunits at the Glu-B1 or Glu-D1 allele, were investigated with four levels of protein content. The effect of the addition of subunits 20 at the Glu-B1 to subunits 5+10 at the Glu-D1 allele on bread-making quality was poor. The strength of the dough was only slightly affected despite the increase in protein content. Subunits 2+12, subunits 4+12 and subunits 2.2+12 at Glu-D1 allele had little effect on dough properties when various protein contents were used, compared with subunits 5+10. Subunits 2.2+12 had the most negative effect on the physical properties of the dough at the Glu-D1 allele. These results clearly showed that each HMWG subunit affected the properties of the dough differently, according to the increase of protein content.
