Eubacterium limosum JCM6421^T influences quercetin production from isorhamnetin and short-chain fatty acid production from various sugars

Abstract

Isorhamnetin (3′-methylquercetin) is one of the major metabolites of quercetin. We hypothesized that Eubacterium limosum JCM6421 ^T transforms isorhamnetin back to quercetin and the type of sugar affects short-chain fatty acid production by E. limosum JCM6421^T. Anaerobic broth (0.2 mL) containing this bacterium was added to the isorhamnetin solution and anaerobically incubated for 8 and 24 h. Additionally, E. limosum JCM6421^T was anaerobically incubated with various 2 µL-sugar solutions for 24 h and short-chain fatty acid production by this bacterium was measured. Quercetin was produced by anaerobic incubation of isorhamnetin with E. limosum JCM6421^T. Its production increased with an increase in incubation time from 8 to 24 h. Mannitol was the ideal sugar for butyric and acetic acid production by E. limosum JCM6421^T among the investigated sugars. We discovered that E. limosum JCM6421^T produces quercetin from isorhamnetin.