Recently, we have developed a method for the determination of uric acid in mammalian tissues by reversed-phase high-performance liquid chromatography with electrochemical detection.
In addition, we have achieved to simplify the treatment of mammalian tissues prior to chromatography as well as to shorten the time for uric acid separation by the chromatography. In this article, we demonstrate the effectiveness of the chromatographic method showing some chromatograms of mammalian tissues. The analytical recoveries of uric acid in mammalian tissues were found to be almost 100%. The chromatographic analysis was performed in about 8 min. The assay limit was about 10 pg.
