簡易人工ルーメン法による消化率測定の迅速化

抄録

A simplified in vitro rumen procedure for the determination of digestibility was proposed. The method was developed on the basis of Van Soest method, in which the acid pepsin treatment of Tilley and Terry method was replaced by a cell-wall determination using the neutral detergent procedure. In this instance, laborious manipulations were necessary to transfer the contents of the incubation glass tube to a container for refluxing with 100ml of the neutral detergent solution. In the simplified method, on the other hand, 20ml of the neutral detergent of 5 times concentration which contains the solutes in equal amounts with 100ml of the solution which used in the ordinary procedure, 1ml of decalin and 0.3g of sodium sulfite were added directly into the incubation tube and the tube was boiled for 1hr in a waterbath. The digestibility of three forages were determined by Tilley and Terry method, Van Soeat method and the simplified method, respectively. Standard deviations of the means obtained by the simplified method were a little larger than Van Soest method, but smaller than Tilley and Terry method. Highly significant correlation was observed between the obtained values by the simplified method and by Van Soest method in respective nine species (r=0.998, P<0.01). Comparing those methods, it was found that the simplified method required less time and fewer manipulations and gave digestibility with a high accuracy. It may be concluded that this method will be of aid to plant scientists who want to know the digestibility of many samples at a time.