DNA Fingerprinting of Selected Maize (Zea mays L.) Genotypes using SSR Markers

抄録

Molecular marker has been used for variety identification, genetic diversity of genotypes and additional using intellectual property protection in DUS testing. DNA fingerprinting of fourteen maize genotypes had been studied at Plant Biotechnology Laboratory, DAR, Myanmar during 2020-2021. Nine hybrids and five inbreds were analyzed using 24 polymorphic SSR markers, resulted a total number of 101 alleles with a range from 2 to 9 alleles per locus. Polymorphism has been sufficiently detected with the average of 0.66 per SSR locus. Cluster analysis separated all the maize genotypes as five major groups and indicated the existence of genetic variation among the observed inbreds and hybrids. Six SSR primer pairs (dupssr12, bnlg1940, umc1248, umc1586, bnlg1518 and bnlg1028) were selected as final marker set for variety identification with the selection criteria such as detection rate of the SSR fragment, the presence of rare allele, PIC value, and reproducibility and PCR band pattern of SSR fragments. In this study, all tested genotypes have been fingerprinted with unique profile identity (ID) to support the DNA fingerprint catalogues of Myanmar Maize Molecular DUS test guidelines.