Journal of Applied Glycoscience
Online ISSN : 1880-7291
Print ISSN : 1344-7882
ISSN-L : 1344-7882
Regular Papers
Immobilization of α-Glucuronidase Specific for O-α-D-Glucosyluronic Acid α-D-Glucosiduronic Acid from Aspergillus niger and Production of D-Glucuronic Acid
Takaaki KiryuHirofumi NakanoTaro KisoHiromi Murakami
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2006 Volume 53 Issue 3 Pages 181-185

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Abstract

α-Glucuronidase from Aspergillus niger specific for O-α-D-glucosyluronic acid α-D-glucosiduronic acid (trehalose dicarboxylate, TreDC) was hydrolyzed TreDC to produce D-glucuronic acid. For the application of the α-glucuronidase to D-glucuronic acid production, the enzyme was immobilized on a baked diatomite. The immobilized enzyme treated with glutaraldehyde retained about 60% of the initial activity after repeated uses of 20 cycles. Although the immobilized enzyme completely hydrolyzed 100 mM TreDC, the reaction was significantly inhibited by D-glucuronic acid over 200 mM TreDC. To hydrolyze TreDC completely, the reaction mixture was diluted with buffer to keep the D-glucuronic acid concentration below 300-400 mM. The immobilized enzyme in the diluted reaction mixture hydrolyzed 500 mM TreDC (initial concentration) almost completely to produce D-glucuronic acid. The immobilized enzyme packed in a column continuously produced D-glucuronic acid and the activity of the enzyme remained about 80% of the initial activity after 20 days of operation.

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© 2006 by The Japanese Society of Applied Glycoscience
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