Structural Analysis of a Novel Trisaccharide Containing Fructopyranoside Residue Isolated from a Fermented Beverage of Plant Extracts

Abstract

Super Ohtaka^® (fermented beverage of plant extracts) is prepared from approximately 50 vegetables and fruits. Natural fermentation is primarily performed using lactic acid bacteria (Leuconostoc spp.) and yeast (Zygosaccharomyces spp.). An unidentified oligosaccharide was isolated from this beverage using carbon-Celite^® column chromatography and high performance liquid chromatography. The oligosaccharide structure was confirmed by MALDI-TOF MS and NMR measurements. This oligosaccharide was identified as β-D-fructopyranosyl-(2→6)-β-D-glucopyranosyl-(1→6)-D-glucose, it was newly found from natural source. We speculated that this oligosaccharide was generated or enhanced during the fermentation, and was barely degraded by artificial gastric juice and rat intestinal enzymes, although it was slightly hydrolyzed by pig pancreatic enzymes.