抄録
Vitamin K1 was separated by thin-layer chromatography with silicagel (gips 5%) using n-hexane-chloroform (20 : 10) as a solvent system. The V. K1 extracted with ethylacetate from the spot of the thin-layer were irradiated with ultra violet ray to be converted into fluoresent substances. Thus 0.5∼5 μg/ml of V. K1 can be estimated quantitatively by measuring the strength of fluorescence.
