Abstract
The paper electrophoretic method for separation and identification of monobasic n-saturated lower fatty acids (C1 to C10) was investigated. The fatty acids were separated by using 3% aqueous ammonia or the tenfold diluted of Atkins-Pantin buffer solution (pH 10) as electrolytes, and detected by staining with bromocresol green and bromothymol blue. For the electrolytes, 3% aqueous ammonia was superior to the Atkins-Pantin buffer solution in the separation of propionic and butyric acids, or caprylic and pelargonic acids.
