Abstract
Analgesic components (5-allyl-5-isobutylbarbituric acid, caffeine, and aminopyrine) in a tablet and biological materials were determined by gas-liquid chromatography with hydrogen flame ionization detector on 1.5 m glass column packed with 3.5% SE-30 at 190°. Determination was made successfuly by the internal standard method using methaqualone. These components were extracted from urine with chloroform after adjusting the pH to 8.0 with 10% ammonium hydroxide solution. The cleanup procedure suitable for the extract was carried out by the Florisil column. The components were eluted with ethyl acetate : chloroform (4 : 1) mixture. The recovery rates of allyl-isobutylbarbituric acid, caffeine, and aminopyrine added to urine were 92.9%, 95.1% and 84.0%, respectively.
