リノール酸の酸化分解に及ぼす金属および金属-EDTAの影響

抄録

A simple method for measurement of linoleic acid by high pressure liquid chromatography was developed to examine the degradation of linoleic acid in the presence of metals and their EDTA complexes. The chromatographic conditions were as follows : Column, Hitachi gel 3010-CH₂OH (20cm×4mm) ; mobile phase, EtOH-0.01 N HCl (9 : 1) ; flow rate, 1 ml/min ; detector, UV (at 254nm). The degradation rate of linoleic acid was examined under following conditions : A mixture of 2.9ml of 0.05M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid) buffer (pH 7.2), 1.0 ml of 3 mM EDTA or distlled water, and 0.1 ml of 3 mM metal solution was placed in a test tube, 0.1 ml of 0.1 M linoleic acid in EtOH was added and the mixture was kept for 24 hr at 25°. A portion of the mixture (20 μl) was injected into the liquid chromatograph to measure residual linoleic acid. It was found that Mn²⁺, Cu²⁺, Fe³⁺ and Fe³⁺-EDTA remarkably accelerated the oxidative degradation of linoleic acid, while Mn²⁺-EDTA suppressed the degradation.