抄録
The liver of Cyprinus carpio was capable of metabolizing benzo [α] pyrene to 3-hydroxybenzo [α] pyrene. The enzyme system, localized in the 105000×g sedimented pellet (microsomal fraction) of liver homogenate, has an absolute requirement for NADPH, and a partial requirement for MgCl2. The enzyme activity was detected in the liver and kidneys, but not in the gill, intestine, heart, or spleen of the fish. The enzyme was inhibited by KCN, NaN3, cytochrome c, and SKF 525-A, which are known to be the inhibitor of hepatic monoxygenase in mammals. It was also strongly inhibited by 7, 8-benzoflavone and vitamin A which are known as inhibitors of the mammalian cytochrome P 448-linked oxidative enzyme system.
