Analysis of Mixed Pharmaceutical Preparations by High Speed Liquid Chromatography. V. Determination of Antipyretics and Antihistaminics in Anti-cold Drugs by High Speed Liquid Chromatography

Abstract

A rapid and sensitive high-speed liquid chromatographic assay was developed for acetaminophen, ethoxybenzamide, caffeine, and chlorpheniramine maleate in anti-cold drugs. Acetaminophen, ethoxybenzamide, and caffeine were separated by injection of methanol solution into the column (50 cm×3 mm) packed with Hilachi Gel 3011 (styrene-divinylbenzene copolymer) and eluted with mixture of methanol-28% ammonia water (99 : 1) and detected at 280 nm. Chlorpheniramine maleate was separated by injection of aqueous solurion into the column (50 cm×3 mm) packed with Hitachi Gel 3011, eluted with a mixture of acetonitrile-28% ammonia water (99 : 1), and detected at 225 nm.