Analysis of Mixed Pharmaceutical Preparations by High Speed Liquid Chromatography. VI. Determination of Antipyretics by High Speed Liquid Chromatography

Abstract

A rapid and sensitive high-speed liquid chromatographic assay was developed for aspirin, aspirin aluminium, salicylic acid, acetaminophen, aminopyrine, and sulpyrine in antipyretic preparations. Aspirin, salicylic acid, acetaminophen, aminopyrine, and sulpyrine were separated by injection of the solution into a column (50 cm×3 mm i.d.) packed with Hitachi Gel 3011. Aspirin, salicylic acid, and acetaminophen were eluted with methanol-glacial acetic acid (99 : 1) and detected at 280 and 310 nm. Aminopyrine was eluted with methanol-28% ammonia water (99 : 1) and detected at 280 nm. Aspirin aluminium was dissolved in methanol-phosphoric acid (99 : 1) at 50°to prepare a sample solution.