イオン会合性試薬を用いたスクリーニングテストにおけるメタンフェタミンの抽出率

抄録

The ion association reagents are used for screening test of methamphetamine in the human urine. The ion-associate of methamphetamine with bromocresol green (BCG), chrome azurol S (CAB), eriochrome Black T (EBT), erio green B (EGB), orange IV (AY), or zincon (Z) is extracted with organic solvent. The color of ion-associate in organic solvent suggests the presence of methamphetamine. Then, the extraction rate (recovery percentage) of ion-associate in organic solvent is examined by gas chromatography. The experimental procedure is as follows : Two ml of organic solvent containing ion-associate is added to 1 ml of a mixture of hydrochloric acid and methanol (1 : 4), and evaporated on hotplate at 150°C. To the residue is added 0.5 ml of trifluoroacetic anhydride, and the trifluoroacetyl derivatives of methamphetamine is quantitatively analyzed by gas chromatography. The result indicated that 4-methyl-2-pentanone was the best reagent for the extraction of methamphetamine, although this solvent was colored by urine sample which did not contain methamphetamine. The best organic solvents for the reagent in screening test of methamphetamine in the urine are dichloromethane for BCG, CAB, EBT, and EGB, 1, 2-dichloroethane for AY, and chloroform for Z, respectively.