Abstract
Methamphetamine was found to form an ion-associated complex with rose bengal (RB) by shaking with 1 ml of 0.1% RB solution in the presence of bromphenol blue to prevent the interfering of RB layer. The complex was successfully extracted with dichloromethane at pH 7.3 and gave yellowish orange fluorescence under UV light. The limit of detection of methamphetamine determined by the aid of the fluorescence of the extracted layer was 0.2 μg in aq. solution and 0.6 μg in the urine. This method was applied to the screening test for urine specimens of methamphetamine-abuse suspects and satisfactory results were obtained.
