1988 年 34 巻 3 号 p. 237-240
By the use of reversed phase high performance thin layer chromatography, most cannabis acids were able to be separated from neutral cannabinoids (CN) simultaneously. Therefore, the following field-test for cannabis has been developed ; cannabis were extracted with acetone for 2-3 min, developed in acetonitrile for 5 min, dipped in a color-producing reagent where Echtblausalz B was suspended in a solution of sodium hydroxide for one second. This method made it possible to distinguish clearly the types of cannabis with different CN, CBDA species, native varieties and hybridized varieties. Moreover, the results agreed very closely with those obtained by the conventional GC method.