直接試料導入/電子衝撃イオン化質量分析法による尿中1,4-ベンゾジアゼピン系薬物のスクリーニング法

抄録

A screening method for 1, 4-benzodiazepines and their metabolites in the human urine was investigated by direct inlet-electron impact mass spectrometry (DI/EI). The 1, 4-benzodiazepines and their metabolites were extracted with ethylether from the urine. The extract was evaporated to dryness and the dried residue was dissolved in 0.2 N hydrochloric acid. The solution was neutralized with 2 N sodium hydroxide solution and applied to the Sep-pak C₁₈ cartridge with dichloromethane-methanol (9 : 1) as eluent. The eluate was evaporated to dryness, dissolved in methanol. Methanol solution was analyzed by mass chromatography (MC) with selected specific fragment ion in DI/EI of the 1, 4-benzodiazepines and their metabolites. The 1, 4-benzodiazepines and their metabolites less than 20 ng were well-detected by DI/EI/MC.