Abstract
The distribution patterns of thiobarbituric acid (TBA) reactive substances and vanadium in subcellular fractions of the liver and kidney of the mice administered with sodium vanadate were investigated at 3 h and 12 h after i.p. injection. The results obtained are as follows. (1) Among subcellular fractions of the liver and kidney, a remarkable enhancement of TBA reactive substances after the administration of vanadium was recognized in the liver supernatant fractions. That is, the elevation of TBA value in the liver supernatant at 3 h and 12 h after the administration were 21.8 and 13.4 times as large as the control one, respectively. (2) From the results of Sephadex G-75 gel chromatography, it was considered that TBA reactive substances in the liver supernatant exist at the high-molecular-weight fractions (molecular weight of 75000 or thereabout) and low-molecular-weight fractions (molecular weight of nearly 1500). Vanadium was found mainly at the eluated fractions of TBA reactive substances. But, vanadium in the low-molecular-weight fractions decreased rapidly. (3) Pretreatment with ascorbic acid did not suppress the formation of lipoperoxide in the liver supernatant after the administration of vanadium.
