フェノキシ系除草剤2,4-Dichlorophenoxy acetateのガスクロマトグラフィーによる裁判化学的研究

抄録

Accidental and suicidal poisonings with sodium 2, 4-dichlorophenoxy acetate (2, 4-D-Na) have occurred occasionally in the fields of forensic toxicology. The gas chromatographic method for the determination of 2, 4-D-Na involving the preparation of the n-propyl ester was studied. 2, 4-D-Na was directly converted with n-propanol-boron-trifluoride to the n-propyl ester which was measured by gas chromatography, although the esterifications so far have been carried out after being led to its free acid. Sep-pak C₁₈ cartridge was applied to the simple and rapid extraction of 2, 4-D-Na from the urine in the following matter, a urine sample was adjusted to pH 7.0, poured into a Sep-pak C₁₈ cartridge, 2 ml of H₂O-methanol (75 : 25, v/v) was put into the cartridge to selectively eliminate urinary components and finally 2 ml of H₂O-methanol (20 : 80, v/v) was poured into the cartridge to elute 2, 4-D-Na, at a constant rate of flow, 5 ml/min. 2, 4-D-Na was satisfactorily recovered and no interference was observed for gas chromatography after esterification. This method is more efficient, simple, and less time-consuming than previous described methods.