High-Performance Liquid Chromatographic Determination of Triazolam in Biological Fluids

Abstract

We describe the simple, rapid and sensitive quantitation of triazolam in human biological fluids by an HPLC with solid-phase extraction. Triazolam was extracted from human plasma with an excellent recovery with Bond Elut CN-E as a solid-phase extraction column. The extract was then applied to HPLC fitted with a C8 column. The effluent was monitored by UV detection at 230 nm. The validation results indicate that the method showed excellent intra- and inter-assay precision and accuracy with high sensitivity. The low limit of quantitation was 0.2 ng/ml when 2 ml of plasma sample was available. The method was successfully applied to plasma, urine and effluent of gastric lavage collected from patients.